Fig. 4: The depletion of CDX2 antagonized the synergistic effects of the combination of OCA and GSK126 on tumor inhibition. | Cell Death & Disease

Fig. 4: The depletion of CDX2 antagonized the synergistic effects of the combination of OCA and GSK126 on tumor inhibition.

From: Activation of FXR and inhibition of EZH2 synergistically inhibit colorectal cancer through cooperatively accelerating FXR nuclear location and upregulating CDX2 expression

Fig. 4: The depletion of CDX2 antagonized the synergistic effects of the combination of OCA and GSK126 on tumor inhibition.

a The protein levels of CDX2 and FXR (nuclear) in HCT116 and SW480 cells treated with OCA and GSK126 alone or in combination by western blotting analysis (left panel: gel bands; right panel: quantitative analysis of these proteins). b The effect of OCA and GSK126 on the nuclear translocation of FXR detected by IF staining. c, d, e The effect of CDX2 depletion on the growth (c), colony formation (d left panel: colony formation assays; right panel: quantitation of the data) and cell cycle distribution (e left panel: cell cycle analysis assays; right panel: quantitation of the data) of HCT116 and SW480 cells treated with OCA and GSK126 alone or in combination. f, g The effect of CDX2 depletion on the migration (f left panel: wound-healing assays; right panel: quantitation of the data) and invasion (g left panel: transwell assays; right panel: quantitation of the data) of HCT116 and SW480 cells treated with OCA and GSK126 alone or in combination. All data are the mean ± SD of three independent experiments. *P < 0.05.

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