Fig. 5: The combination of OCA and GSK126 synergistically accelerated FXR nuclear localization and promoted CDX2 expression and its regulated signaling pathways. | Cell Death & Disease

Fig. 5: The combination of OCA and GSK126 synergistically accelerated FXR nuclear localization and promoted CDX2 expression and its regulated signaling pathways.

From: Activation of FXR and inhibition of EZH2 synergistically inhibit colorectal cancer through cooperatively accelerating FXR nuclear location and upregulating CDX2 expression

Fig. 5: The combination of OCA and GSK126 synergistically accelerated FXR nuclear localization and promoted CDX2 expression and its regulated signaling pathways.

a The protein levels of AKT/p-AKT (Ser473), GSK-3β/p-GSK-3β (Ser9), and β-Catenin (Nuclear) in HCT116 and SW480 cells treated with OCA and GSK126 alone or in combination by western blotting analysis (left panel: gel bands; right panel: quantitative analysis of these proteins). b The protein levels of AKT/p-AKT (Ser473), GSK-3β/p-GSK-3β (Ser9) and β-Catenin (Nuclear) in HCT116 and SW480 cells with FXR overexpression, EZH2 depletion alone or in combination by western blotting analysis (left panel: gel bands; right panel: quantitative analysis of these proteins). c, d The effect of CDX2 depletion on the he protein levels of AKT/p-AKT (Ser473), GSK-3β/p-GSK-3β (Ser9), and β-Catenin (Nuclear) in HCT116 and SW480 with OCA and GSK126 combination (c left panel: gel bands; right panel: quantitative analysis of these proteins) or FXR overexpression and EZH2 depletion (d left panel: gel bands; right panel: quantitative analysis of these proteins). e The effect of OCA and GSK126 on the nuclear translocation of β-Catenin detected by IF staining. f The effect of CDX2 depletion on the protein levels of cell cycle-related, apoptosis-related, and EMT-related proteins in HCT116 and SW480 cells treated with OCA and GSK126 alone or in combination by western blotting analysis (left panel: gel bands; right panel: quantitative analysis of these proteins). All data are the mean ± SD of three independent experiments. *P < 0.05.

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