Fig. 6: In vivo pharmacological inhibition of voltage-dependent anion channel 1 (VDAC1) using 4,4′-diisothiocyano-2,2′-stilbenedisulfonic acid (DIDS) attenuates propidium iodide (PI) spread in the regions next to the lesion.
From: VDAC1 regulates neuronal cell loss after retinal trauma injury by a mitochondria-independent pathway
PI staining in (A) PBS- and (B) DIDS-treated retinas 2 h after lesion. C Representative image of the analyzed region in PBS- and DIDS-treated retinas and the respective scale showing lower (black/blue) to higher (yellow/white) signal intensity, as generated by ImageJ software. D Integrated signal intensity as a function of radial distance from the lesion. (E) Integrated signal intensity as a function of the logarithm of radial distance from the lesion. F Minimal signal intensity after treatment obtained from the five-parameter logistic (5PL) curve fit (n = 6–10). G Maximal signal intensity after treatment obtained from 5PL curve fit (n = 6–10). H Hill slope obtained from 5PL curve fit (n = 6–10). I Distance from the focus of the lesion in which the integrated signal intensity corresponds to an inflection point obtained from 5PL curve fit (n = 6–10). J Symmetry was obtained from 5PL curve fit (n = 6–10). *P < 0.05; **P < 0.01 in Student’s T-test. Scale bar: 100 μm.
