Fig. 6: TXNIP silencing prevents GSDMD cleavage in vitro microglia and in vivo 5xFAD.
From: RAGE-TXNIP axis drives inflammation in Alzheimer’s by targeting Aβ to mitochondria in microglia
A IHC analysis of GSDMDC1 (green) and Iba1 (red) in 4 m old control and 5xFAD mice hippocampus treated as indicated (n = 4). B, C Western blot of native gel (B) and quantification (C) of GSDMD cleavage in wt and 5xFAD mice hippocampus treated as indicated. Total GSDMD and actin were used to normalize. One-way ANOVA followed by Tukey’s multiple comparison test (n = 3; ***p < 0.001 versus control; ##p < 0.01 versus siScramble). D, E Western blot of native gel (D) and quantification (E) of GSDMD cleavage in LPS-primed primary microglia treated as indicated. Total GSDMD and actin were used to normalize. One-way ANOVA followed by Tukey’s multiple comparison test (n = 3; ****p < 0.0001 versus control; ####p < 0.0001 versus siScramble).
