Fig. 5: HspBP1 regulates Rad51 foci in response to IR in breast cancer cells. | Cell Death & Disease

Fig. 5: HspBP1 regulates Rad51 foci in response to IR in breast cancer cells.

From: HspBP1 is a dual function regulatory protein that controls both DNA repair and apoptosis in breast cancer cells

Fig. 5

A, B HspBP1-depleted non-malignant breast cancer cells (MCF10A) or BRCA1-proficient breast cancer cells (MCF7, and MDC-MB-231) and corresponding control cell lines were irradiated with 5 Gy of IR and fixed at 1 h after irradiation. Immunofluorescence analysis with antibodies against Rad51 was then carried out. Nuclei were stained with DAPI. Representative images (A) and the percentage of cells with more than 5 foci for Rad51(B) are shown. Data represent mean ± SD (n = 3), **P < 0.01, two-tailed Student’s t-test. C The levels of HspBP1 in control and BRCA1-reconstituted HCC1937 cells transfected with either control or HspBP1 siRNA. D, E The levels of IR-induced Rad51 foci were measured in control and BRCA1-reconstituted HCC1937 cells transfected with either control or HspBP1 siRNA as described in (A). Representative images (C) and quantification (D) of Rad51 foci are shown. Data represent mean ± SD (n = 3), **P < 0.01, two-tailed Student’s t-test. F, G The levels of IR-induced Rad51 foci were measured in GFP-HspBP1-expressing MCF7, MDC-MB-231, MDA-MB-436 cells and the corresponding control cells as described in (A). Representative images (F) and quantification (G) of Rad51 foci are shown. Data represent mean ± SD (n = 3), **P < 0.01, two-tailed Student’s t-test.

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