Fig. 6: HspBP1 contributes to IR resistance. | Cell Death & Disease

Fig. 6: HspBP1 contributes to IR resistance.

From: HspBP1 is a dual function regulatory protein that controls both DNA repair and apoptosis in breast cancer cells

Fig. 6

A Control and HspBp1-depleted U2OS, MCF-10A, MCF-7, MDA-MB-231, and MDA-MD-436 cells were either untreated or treated with the indicated doses of IR. The viability of untreated and treated cells were examined using the clonogenic survival assay. Results are shown as the mean ± SD (n = 3), **P < 0.01, *P < 0.05, ns, not significant. two-tailed Student’s t-test. B γ-H2AX foci of the same cells as described in (A). Cell were treated with 5 Gy of IR, fixed at 24 h, and immunostained using an anti-γ-H2AX antibody. The percentage of cells with more than 5 γ-H2AX foci is shown. Results are shown as the mean ± SD (n = 3), **P < 0.01, ns Not significant, two-tailed Student’s t-test. C Control and HspBP1-GFP-expressing MCF7, MDA-MB-231, and MDA-MB-436 cells were treated with 5 Gy of IR, fixed at 12 h after, and immunostained using antibody against γ-H2AX. The percentage of cells with more than 5 foci for γ-H2AX is shown. Results are shown as the mean ± SD (n = 3), **P < 0.01, ns Not significant, two-tailed Student’s t-test. D A clonogenic survival assay of the same cells described in (C). Cells were either untreated or treated with the indicated doses of IR. Results are shown as the mean ± SD (n = 3), **P < 0.01, *P < 0.05, ns Not significant, two-tailed Student’s t-test.

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