Fig. 7: Mammary gland tumor-related gene expression signature.
From: Evolution of gene expression signature in mammary gland stem cells from neonatal to old mice
A, B Increased expression of the representative oncogenes including DAD1, CKS2, MCTS1, and SNHG1 (A), and decrease of tumor suppressor genes including RASSF1, SMARCB1, RAF1, and MOAP1 (B) at D180, compared to any younger time points. C GSEA analysis revealed that genes in Cluster 4 are significantly enriched in a mammary gland tumor-related gene signature derived from GSE130472. D Gene mutations at different time points compared to D4 were called by GATK and Varscan2. Sankey diagram showed the number of variants at different time points, in which different colors represent different mutation patterns. The frequency of mutations at each time point was indicated below the Sankey diagram. E Analysis using a cell type-based signature derived from GSE130472 (left panel) indicated the highest proportion of basal cells at D4. While both luminal progenitor and mature luminal cells showed the lowest proportion at D4, and maintained at a high level from D20 to D180 (right panel). DEG differentially expressed gene. Data are presented as the mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.
