Fig. 2: CLDN4 enhances the proliferation, migration and invasion capabilities of GBM cells in vitro.

A The CLDN4 expression was determined after transfecting cells with corresponding vectors by western blotting in U87 and LN229 cells. B The cell proliferation activity was examined by EdU assays. (200×). C The migration ability was determined by wound healing. D The invasion ability was determined by Transwell assay. E The EMT markers were determined by western blotting. Data are represented as the mean ± standard deviation of three independent experiments. Student’s t-test, compared to the Control group. *p < 0.05, **p < 0.01.