Fig. 5: FABP4 knockdown inhibited inflammation and apoptosis in LPS-stimulated TCMK-1 cells. | Cell Death & Disease

Fig. 5: FABP4 knockdown inhibited inflammation and apoptosis in LPS-stimulated TCMK-1 cells.

From: Fatty acid-binding protein 4 is a therapeutic target for septic acute kidney injury by regulating inflammatory response and cell apoptosis

Fig. 5: FABP4 knockdown inhibited inflammation and apoptosis in LPS-stimulated TCMK-1 cells.

TCMK-1 cells were transfected with negative control (NC) siRNA or FABP4 siRNA for 24 h and then treated with 100 μg/ml LPS for another 24 h. The knockdown efficiency of FABP4 siRNA in TCMK-1 cells was evaluated by (A) quantitative real-time PCR analysis and (B) western blot analysis. C Representative western blot images and quantitative analysis of FABP4 in TCMK-1 cells. D The mRNA levels of NGAL and KIM1 detected by quantitative real-time PCR. Western blotting and densitometry quantification of (E) inflammatory factors including IL-1β, IL-6, TNF-α, and MCP-1, and (F) apoptotic marker including Bcl-2, Bcl-XL, Bax, caspase 3 (Cas 3), and cleaved-caspase 3 (C-Cas 3) in TCMK-1 cells. G Representative flow cytometric plots of TCMK-1 cell apoptosis and (H) quantification of apoptosis rate. All data are displayed as mean ± SD (n = 3); **P < 0.01, ***P < 0.001, ****P < 0.0001 versus NC siRNA; #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 versus LPS + NC siRNA.

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