Fig. 4: circFAM120B inhibits the tumorigenicity of ESCC by binding with miR-661 to restore PPM1L expression.

A Proliferation of ESCC cells co-transfected as indicated was assessed by CCK-8 assay (n = 5 biologically independent replicates). B Colony formation assays were performed in ESCC cells co-transfected as indicated (n = 3 biologically independent replicates). C Detection of the proliferating ESCC cells with circFAM120B overexpression or knockdown by EdU assay (n = 3 biologically independent replicates). D Migration and invasion of ESCC cells co-transfected as indicated were examined by Transwell assays (n = 3 biologically independent replicates). E Expression of PPM1L was examined by qRT-PCR in ESCC cells transfected as indicated. F–H The expression levels of specific proteins were analyzed by western blot analysis in ESCC cells treated as indicated. *P < 0.05, **P < 0.01, ***P < 0.001.