Fig. 1: Immunosupportive effect of LNMSCs.
From: Mesenchymal stem cells empower T cells in the lymph nodes via MCP-1/PD-L1 axis
A The distribution of Gli1-positive MSCs in the lymph nodes and bone marrow of Gli1-CreERT2; R26tdTomato mice. Scale bars = 200 μm for lower magnification and 20 μm for higher magnification. B Schematic figure of the distribution of Gli1-positive MSCs in mouse lymph nodes and bone marrow. C Live-cell counting of trypan blue-positive T cells after co-culture with LNMSCs and BMMSCs. n = 3. D, E CFSE analysis and EdU staining showed that LNMSCs promote activated T-cell proliferation. Scale bar = 50 μm. n = 7–9 (D), n = 3–5 (E). F Flow cytometry analysis showed the anti-apoptotic effect of LNMSCs on activated T cells. n = 4–5. G Flow cytometry analysis showed that LNMSCs stimulate CD4+ T-cell activation. n = 3-4. H–K Flow cytometry analysis showed that LNMSCs and BMMSCs regulated T-cell differentiation. n = 4 (H), n = 3–4 (I), n = 3 (J), n = 3–4 (K). *P < 0.05, **P < 0.01, ***P < 0.001.
