Fig. 4: Flubendazole-induced mitophagy inhibits cell proliferation in flubendazole-treated MDA-MB-231 and MCF-7 cells. | Cell Death & Disease

Fig. 4: Flubendazole-induced mitophagy inhibits cell proliferation in flubendazole-treated MDA-MB-231 and MCF-7 cells.

From: Flubendazole induces mitochondrial dysfunction and DRP1-mediated mitophagy by targeting EVA1A in breast cancer

Fig. 4

A MDA-MB-231 and MCF-7 cells were transfected with negative-control or DRP1 shRNA for 24 h, respectively. After treatment with or without flubendazole (0.5 μM) for 24 h, cell viability was measured by MTT assay. B, C MDA-MB-231 and MCF-7 cells were transfected with negative-control or DRP1 shRNA for 24 h, respectively. After treatment with or without flubendazole (0.5 μM) for two weeks. Representative images and quantification of colonies were shown. D–F MDA-MB-231 and MCF-7 cells were transfected with negative-control or DRP1 shRNA for 24 h, respectively. After treatment with or without flubendazole (0.5 μM) for 24 h, then detected by Edu assay (D, E) and LDH release (F). Scale bar, 50 µm. Data represent mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. Statistical significance compared with respective control groups (all P-values were obtained by one-way ANOVA).

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