Fig. 1: Nhe1 Astro-KO brains displayed preserved neuronal GRP78 expression and NeuN+ cell counts after ischemic stroke.
From: NOX activation in reactive astrocytes regulates astrocytic LCN2 expression and neurodegeneration
A Experimental protocol and timeline. B Representative immunofluorescence images of GRP78 protein expression in wild-type and Nhe1 Astro-KO brains at 48 h Rp. High magnification images show peri nuclear GRP78 staining in peri-lesion areas indicated by white squares. C Representative confocal images of NeuN and GRP78 staining in neurons. Arrows: high GRP78 protein expression. Arrowheads: low GRP78 expression. D, E Summary data of GRP78+ and NeuN+ cells. Data are mean ± SD, n = 4. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 via one-way ANOVA. CL contralateral, IL Ipsilateral hemispheres.
