Fig. 2: Evaluation of visual function in WT and OPTN (E50K) mice.

A, B F-VEP was used to access the light reflex in WT and E50K mice. (WT-3M, 21.2 ± 1.6, n = 31, E50K-3M, 21.6 ± 1.9, n = 21, WT-16M, 17.1 ± 0.7, n = 41, E50K-16M, 12.4 ± 0.7, n = 43). C With a designed Light/Dark Transition apparatus, the length of time the free-roaming mice spent in light space was monitored. (WT-3M, 188.8 ± 9.9, n = 17, E50K-3M, 183.6 ± 18.3, n = 10, WT-16M, 175.2 ± 18.3, n = 22,E50K-16M, 234.4 ± 12.9, n = 31). D–F With a designed OMR test apparatus, the head movement was captured to indicate the response to object movement at different frequencies in the environment. The top-view photograph of the OMR system consisted of a visual stimulus unit, which displays clockwise rotation of black-and-white stripes on 4 LCD screens (D, E). The number of OMR under each condition was analyzed (F). ①0.05 cpd: WT-3M, 10.5 ± 0.5, n = 36, E50K-3M, 12.1 ± 0.9, n = 11, WT-16M, 11.4 ± 0.62, n = 13, E50K-16M, 3.6 ± 0.4, n = 16. ②0.1 cpd: WT-3M, 9.4 ± 0.5, n = 36, E50K-3M, 8.9 ± 0.8, n = 13, WT-16M, 8.8 ± 0.8, n = 19, E50K-16M, 4.5 ± 0.4, n = 16. ③0.2 cpd: WT-3M, 9.3 ± 0.7, n = 24, E50K-3M, 8.2 ± 0.8, n = 10, WT-16M, 8.2 ± 0.8, n = 17, E50K-16M, 3.8 ± 0.3, n = 14. (*P < 0.05, **P < 0.01, ***P < 0.001).