Fig. 7: Wnt5a-FKO mice after 20 weeks on the HFD.

Floxed and Wnt5a- FKO mice were fed the ND or the HFD for 20 weeks from 8 weeks old. A Comparison of the body weights of Floxed or FKO mice on the normal chow diet (ND) or the high fat diet (HFD) for the indicated periods. Mice were weighed at fortnightly (Floxed, ND, n = 4; FKO, ND, n = 3; Floxed, HFD, n = 6; FKO, HFD, n = 6). B The total fat, free body fluid, and lean body masses were analyzed by nuclear magnetic resonance (Floxed, ND, n = 4; FKO, ND, n = 4; Floxed, HFD, n = 6; FKO, HFD, n = 6) after 8 weeks on the ND or the HFD. (C) Weights of sWAT, eWAT, liver, spleen, and BAT of Floxed and FKO mice (Floxed, ND, n = 5; FKO, ND, n = 4; Floxed, HFD, n = 6; FKO, HFD, n = 6). D–F Food intakes (D), activities (E), and VO₂ values (F) as determined by indirect calorimetry for Floxed and FKO mice (Floxed, ND, n = 4; FKO, ND, n = 4; Floxed, HFD, n = 6; FKO, HFD, n = 6) after 8 weeks on the ND or the HFD. (G) Serum biochemistry of lipid parameters in overnight-fasted Floxed and FKO mice (Floxed, HFD, n = 6; FKO, HFD, n = 6). (H) GTT and AUC analysis. Blood glucose concentrations of mice after 9 weeks on the ND or HFD were measured at various times after glucose (1.5 g/kg) treatment. Areas under the curve (AUC) for intraperitoneal glucose tolerance test results were calculated (Floxed, ND, n = 5; FKO, ND, n = 5; Floxed, HFD, n = 6; FKO, HFD, n = 7). I Relative mRNA expression levels of IL-6, TNFα, MCP1, and F4/80 in sWATs of Floxed and FKO mice (Floxed, HFD, n = 6; FKO, HFD, n = 6). mRNA levels were normalized versus 18 S rRNA. Data are the means ± SEMs of three independent determinations. The significances of differences were determined using a two-tailed paired Student’s t-test. *p < 0.05, **p < 0.01.