Fig. 5: R2 causes retention of WTErbB-2 and ErbB-2c in different intracellular compartments. | Cell Death & Disease

Fig. 5: R2 causes retention of WTErbB-2 and ErbB-2c in different intracellular compartments.

From: Halting ErbB-2 isoforms retrograde transport to the nucleus as a new theragnostic approach for triple-negative breast cancer

Fig. 5

A Trastuzumab binding was determined in cells treated with R2 (100 µM) or vehicle for 24 h. Representative histograms. Quantification of trastuzumab binding by delta MFI (n = 2, mean ± SD). Left barplot: cells in control conditions (DMSO); right barplot: MFI of treated cells. B Cells were pretreated with R2 (100 µM) or vehicle and then with trastuzumab (10 µg/ml) for analysis of proliferation by cell count or [3H]-thymidine incorporation. C−F, Colocalization of ErbB-2 with E-cadherin (C), EEA1 (D), GalNAc-T-Cherry (E), and calnexin (F) was analyzed by IF in cells treated as in A. Colocalization analysis was performed as in Fig. 1G. The yellow line indicates the plane used for line profile generation (see Supplementary Materials and Methods). Experiments shown in (B–F): n = 3.

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