Fig. 7: The UUO-induced expression of Col I&III and ɑ-SMA was attenuated due to the inhibition of autophagy in PT-ATG7-KO mice.
From: Proximal tubular RAGE mediated the renal fibrosis in UUO model mice via upregulation of autophagy
The left ureter of PT-ATG7-KO and PT-ATG7-WT littermate mice was ligated for 7 days to establish a UUO model. Immunoblotting results demonstrated that the UUO induced the increasing of fibronectin & Col I&III, Vimentin, but the expression of them was reduced due to the downregulation of autophagy in PT-ATG7-KO mice. A Immunoblot analysis of Atg7, LC3, p62, and fibronectin & Col I&III, Vimentin. B–H Analysis of the grayscale image between them. Data are expressed as means ± s.d. (n = 6). #P < 0.05 versus PT-ATG7-WT with the sham group. *P < 0.05 versus PT-ATG7-WT with the UUO group. Each experiment (A) was repeated six times independently with similar results. B–H indicate the statistical Student’s t test used.
