Fig. 3: Obatoclax is a potent inducer of apoptosis and oxidative stress in AT/RT. | Cell Death & Disease

Fig. 3: Obatoclax is a potent inducer of apoptosis and oxidative stress in AT/RT.

From: Dual mTORC1/2 inhibition compromises cell defenses against exogenous stress potentiating Obatoclax-induced cytotoxicity in atypical teratoid/rhabdoid tumors

Fig. 3: Obatoclax is a potent inducer of apoptosis and oxidative stress in AT/RT.

A MUSE oxidative stress assay. M1 represents reactive oxygen species (ROS) negative cells, M2 ROS-positive cells. DMSO-treated cells shaded in gray, Obatoclax ROS negative (blue), ROS positive (red). B Graphs illustrating percent of cells ROS positive. The results are presented as the mean +/− SEM. **Indicates p < 0.005, t-test. C Muse oxidative stress assay. CHLA06 cells were treated with the IC50 dose of the BH3 mimetics Navitoclax and Venetoclax, and the MCL-1 inhibitors AZD5991 and S63845. ROS-negative cells (blue), ROS- positive cells (red). DMSO-treated cells shaded in gray. D Western blot 24 h after Obatoclax treatment in 4 AT/RT cell lines probing for cleaved PARP (cPARP) and the anti-apoptotic proteins MCL-1 and BCL-xL. Numbers above blots represent quantification of protein expression normalized to ACTIN. E Diagram illustrating complementary mechanisms of action between TAK-228 and Obatoclax. TAK-228 interferes with cell defenses against apoptosis and oxidative stress, while Obatoclax induces apoptosis and oxidative stress in AT/RT.

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