Fig. 6: Expression of GluN1, GluN2A and GluN2B in total homogenates and the PSD after the behavioral evaluation.
From: SAPAP3 regulates epileptic seizures involving GluN2A in post-synaptic densities
A–C Representative western blot of the GluN2A levels in total tissue homogenates and subcellular fractions (A). No significant differences in the total GluN2A levels were found among the groups (B). Compared with the control group, the SAPAP3-sh vector significantly decreased the synaptic/total GluN2A ratio. SAPAP3 overexpression significantly increased the synaptic/total GluN2A ratio (C). Representative western blots of the GluN2B (D) and GluN1 levels (G) in total tissue homogenates and subcellular fractions. No significant differences in the total GluN2B and GluN1 levels were found among the groups (E and H). The synaptic/total GluN2B and GluN1 ratios were not significantly different compared with those of the control group (F and I). GAPDH served as a loading control for the total homogenates. Data are presented as means ± SEM, n = 4 per group. *p < 0.05; ***p < 0.001.
