Fig. 3: The effects of UHMK1 upregulation on CRC cell proliferation, tumorigenicity and oxaliplatin resistance. | Cell Death & Disease

Fig. 3: The effects of UHMK1 upregulation on CRC cell proliferation, tumorigenicity and oxaliplatin resistance.

From: UHMK1 aids colorectal cancer cell proliferation and chemoresistance through augmenting IL-6/STAT3 signaling

Fig. 3: The effects of UHMK1 upregulation on CRC cell proliferation, tumorigenicity and oxaliplatin resistance.

A Western blot results of UHMK1 overexpression in DLD-1 and SW620 cells. Anti-UHMK1 (left) and anti-FLAG (right) antibodies were used. B The growth rates of DLD-1 and SW620 cells with or without UHMK1 overexpression were monitored by CCK-8 assay. C EdU staining was used to detect cell proliferation in DLD-1 cells with UHMK1 stable overexpression and control cells. The number of positive EdU cells was recorded. Scale bar: 100 μm. D The image of tumors removed from nude mice implanted with DLD-1/UHMK1-2 cells (n = 8) and control RKO cells (n = 8) for 3 weeks. Tumor weight and tumor volume were analyzed compared to control group. E Colony formation assays in UHMK1 stably overexpressed DLD-1 cells and control cells with or without oxaliplatin treatment (100 μM). F FACS analysis of apoptotic DLD-1 cells with or without oxaliplatin treatment (300 μM). UHMK1 stable expression cells had an anti-apoptosis effect. *p < 0.05, **P < 0.01.

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