Fig. 5: Overexpression of EphA3 exerts an opposite effect in DU145 and PC-3 cell lines.
From: ADAM10-cleaved ephrin-A5 contributes to prostate cancer metastasis
A The protein content of EphA3 in PC-3(left) and DU145(right) cells after lentivirus infection was detected by Western blotting, ***P < 0.001, **P < 0.01. B Effect of EphA3 on the proliferation of PC-3 and DU145 cells, PC-3/EphA3 vs PC-3/NC, ***P < 0.001, DU145/EphA3 vs DU145/NC, ***P < 0. 001. C The effect of EphA3 on the migration ability of PC-3 and DU145 cells was detected by scratch healing experiment. PC-3/EphA3 vs PC-3/NC, ***P < 0.001,DU145/EphA3 vs DU145/NC, ***P < 0. 001. Scale bar, 100 µm. D Trans-well assay was used to detect the effect of EphA3 on the invasive ability of PC-3 and DU145 cells, PC-3/EphA3 vs PC-3/NC, *P < 0.05,DU145/EphA3 vs DU145/NC, ***P < 0. 001. Scale bar, 100 µm. E Effect of EphA3 on apoptosis of PC-3 and DU145 cells was detected by flow cytometry, PC-3/EphA3 vs PC-3/NC, **P < 0.01,DU145/EphA3 vs DU145/NC, *P < 0. 05. F Effects of ephrinA5-Fc on phosphorylation of EphA3 protein.
