Fig. 5: ZNF334 promotes cell apoptosis, inhibits proliferation and stemness of liver cancer cells in vitro. | Cell Death & Disease

Fig. 5: ZNF334 promotes cell apoptosis, inhibits proliferation and stemness of liver cancer cells in vitro.

From: DNA hypermethylation modification promotes the development of hepatocellular carcinoma by depressing the tumor suppressor gene ZNF334

Fig. 5: ZNF334 promotes cell apoptosis, inhibits proliferation and stemness of liver cancer cells in vitro.The alternative text for this image may have been generated using AI.

A mRNA expression of ZNF334 in different cell lines. B mRNA expression of ZNF334 in overexpression Huh7 cell line (ZNF334-OE) and SNU387 interfering cell line(sh-ZNF334). C Protein expression of ZNF334 in different cell lines. D Protein expression of ZNF334 in overexpression Huh7 cell line (ZNF334-OE) and SNU387 interfering cell line(sh-ZNF334). E Representative images of ZNF334 expression in HepG2, SNU387 (immunofluorescence staining, ×200, blue fluorescence represents nucleus, red fluorescence represents ZNF334). F Cell proliferation curve of ZNF334-OE and ZNF334-NC by CCK8 in Huh7 cell line. G Cell proliferation curve of sh-ZNF334 and sh-NC by CCK8 in SNU387 cell line. H Proliferation ratio of ZNF334-OE and ZNF334-NC by EdU proliferation assay. I Apoptosis ratio of ZNF334-OE and ZNF334-NC by TUNEL apoptosis assay. J Representative images of proliferation of ZNF334-OE and ZNF334-NC by EdU (immunofluorescence staining, ×200, blue fluorescence represents nucleus, red fluorescence represents proliferating cells). K Cell apoptosis of ZNF334-OE and ZNF334-NC by flow cytometry. L Representative images of apoptosis of ZNF334-OE and ZNF334-NC by EdU (immunofluorescence staining, ×200, blue fluorescence represents nucleus, green fluorescence represents nucleus undergoing apoptosis). M Representative images and statistical analysis of ZNF334-OE, ZNF334-NC, sh-ZNF334, and sh-NC by plate clone formation assay. N Representative images and statistical analysis of ZNF334-OE, ZNF334-NC, sh-ZNF334, and sh-NC by sphere formation assay. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001.

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