Fig. 2: miR-188-3p expression increased during senescence and inhibited angiogenesis in vitro.
From: miR-188-3p targets skeletal endothelium coupling of angiogenesis and osteogenesis during ageing
A The expression of miR-188-3p in primary endothelial progenitor cells from the age-indicated male C57BL/6 mice. n = 3 in each group. B Representative images of β-gal staining from HMEC-1 treated with PBS or H2O2. Scale bar = 200 µm. C–G The relative expression of Ink4a-p16 and SASP factors (IL-1, IL-6, IL-8, TNF-a) in the HMEC-1 cells treated with PBS or H2O2. n = 3 in each group. H–K Images of western blotting (H) and quantitative analysis of P53, P-P53, γ-H2AX protein expression levels (I–K) in the HMEC-1 cells treated with PBS or H2O2. n = 3 in each group. L The expression of miR-188-3p in HMEC-1 treated with PBS or H2O2. n = 3 in each group. M Representative images of tube formation assays from the groups transfected with miR-188-3p mimics or miRNA-NC. Scale bar = 500 μm. N, O Quantification of tube junction numbers (N) and total master segment length (O) in the tube formation assays of the indicated groups. n = 3 miR-188-3p mimic-treated groups, n = 4 miRNA-NC-treated groups. P, Q Representative images (P) and wound healing percentage (Q) of the endothelial cell line transfected with miR-188-3p or miRNA-NC in the wound healing assays. Scale bar = 500 μm. n = 3 in each group. R, S Representative images (R) and quantitative analysis (S) of the migrated cells from a Transwell migration assay. Scale bar = 500 μm. n = 4 miR-188-3p-treated group, n = 5 miRNA-NC-treated group. T, U The relative expression of Emcn (T) and CD31 (U) in the HMEC-1 cells transfected with miR-188-3p mimics or miRNA-NC. n = 3 in each group. NC: treated with miRNA-NC, miR-188-3p mimics: treated with miR-188-3p mimics. Data are shown as the mean ± SD. n.s. no significance; *P < 0.05; **P < 0.01; ***P < 0.001 by two-tailed Student’s t test or Welch’s t test.
