Table 1 List of oligonucleotide primer pairs used in the qRT-PCR analysis.
From: miR-188-3p targets skeletal endothelium coupling of angiogenesis and osteogenesis during ageing
Target gene | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|
P16 | ATCCAGGTGGGTAGAAGGTC | CCCCTGCAAACTTCGTCCT |
IL-1 | CATTGAGCCTCATGCTCTGTT | CGCTGTCTGAGCGGATGAA |
IL-6 | ACTCACCTCTTCAGAACGAATTG | CCATCTTTGGAAGGTTCAGGTTG |
IL-8 | ACTGAGAGTGATTGAGAGTGGAC | AACCCTCTGCACCCAGTTTTC |
TNF-a | CCTCTCTCTAATCAGCCCTCTG | GAGGACCTGGGAGTAGATGAG |
Fabp4 | AAGGTGAAGAGCATCATAACCCT | TCACGCCTTTCATAACACATTCC |
Pparg | ATGGTTGACACAGAGATGC | GAATGCGAGTGGTCTTCC |
Sp7 | ATGGCGTCCTCTCTGCTTG | TGAAAGGTCAGCGTATGGCTT |
Alp | CCAACTCTTTTGTGCCAGAGA | GGCTACATTGGTGTTGAGCTTTT |
Runx2 | GAAATGCCTCCGCTGTTATG | AGGTGAAACTCTTGCCTCGTC |
ITGβ3 | GTGACCTGAAGGAGAATCTGC | CCGGAGTGCAATCCTCTGG |
CD31 | AACAGTGTTGACATGAAGAGCC | TGTAAAACAGCACGTCATCCTT |
EMCN | AGCAACCAGCCGGTCTTATTC | AGCACATTCGGTACAAACCCA |
