Fig. 2: DPI treatment lowers mROS and IFN-γ production in differentiated memory-like CD4+ T cells. | Cell Death & Disease

Fig. 2: DPI treatment lowers mROS and IFN-γ production in differentiated memory-like CD4+ T cells.

From: Mitochondrial reactive oxygen is critical for IL-12/IL-18-induced IFN-γ production by CD4+ T cells and is regulated by Fas/FasL signaling

Fig. 2

In vitro differentiated memory-like CD4+ T cells (as in Fig. 1) were stimulated with IL-12/IL-18. A MitoSOX red fluorescence analyzed by flow cytometry showing mROS production in the early time points of IL-12/IL-18 or IL-12/IL-18 + DPI treatment. Statistical significance is represented by symbols # for MFI increase after stimulation compared to 0 time point, or * for MFI decrease after DPI treatment compared to untreated control of each time point. B Flow cytometry analysis of mROS production using MitoROS dye in naïve vs. memory-like cells at 15 min after treatment with IL-12/IL-18 or IL-12/IL-18 + DPI. C Left, Intracellular staining showing the frequency of IFN-γ-producing CD44hi effector/memory T cells at 24 h after IL-12/IL-18 or IL-12/IL-18 + DPI treatment in CD4+ cells. Right, Statistical analysis of observed differences. D Flow cytometry analysis showing surface expression of CD44 at 24 h after IL-12/IL-18 or IL-12/IL-18 + DPI treatment. E Cytokine secretion at 4 h after IL-12/IL-18 treatment in presence of DPI or MitoQ. Pseudo-color plots and histograms are representative of at least three experiments performed. Statistical significance was estimated using unpaired Student’s t test D, one-way ANOVA with post-hoc Tukey test (C, E) or two-way ANOVA with Sidak’s correction for multiple comparison A, B. Graphs show mean ± SD (n = 3 different mice); *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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