Fig. 4: The phosphorylation TOPK S32 was highly expressed in sorafenib resistance RCC cells. | Cell Death & Disease

Fig. 4: The phosphorylation TOPK S32 was highly expressed in sorafenib resistance RCC cells.

From: TOPK/PBK is phosphorylated by ERK2 at serine 32, promotes tumorigenesis and is involved in sorafenib resistance in RCC

Fig. 4

A The IC50 (half maximal inhibitory concentration) of five RCC cell lines for sorafenib were dectected respectively. B The sorafenib sensitive cell line (786-O) was induced to resistance cell line (786-O-SR), the IC50 of the sorafenib in both 786-O and 786-O-SR was detected. C The apoptosis of 786-O, 786-O-SR and ACHN in different concentration of sorafenib was detected by flow cytometry, data were represented as means ± SD of triplicate experiments. *, means P < 0.05, **, means P < 0.01. D 786-O, 786-O-SR, ACHN were cultured with media contained with sorafenib of different concentration for 24 h before harvest. The expressions of p-TOPK (S32) and TOPK were determined by western-blot. E TOPK was knocked down in 786-O-SR with lentiviral infection. F The cells were treated with sorafenib of different concentration (0, 5,10 μM), and flow cytometry was used to determine the cell apoptosis. G The plasmids of pcDNA3, pcDNA3-TOPK and pcDNA3-TOPK-S32A were stable transfected into 786-O cell lines respectively. H The cells were treated with different concentration of sorafenib (0, 5,10 μM), and flow cytometry assay was used to determine the cell apoptosis.

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