Fig. 2: Enumeration of sub-populations of synovial MPCs with abnormal aggrecan staining. | Cell Death & Disease

Fig. 2: Enumeration of sub-populations of synovial MPCs with abnormal aggrecan staining.

From: Synovial mesenchymal progenitor derived aggrecan regulates cartilage homeostasis and endogenous repair capacity

Fig. 2

Intracellular flow cytometry was employed to quantify cells with intense aggrecan staining adjacent to the nucleus within normal vs. OA synovial MPC populations. Overall synovial MPCs from normal and OA donors maintained a similar forward and side-scatter appearance (A), with unstained MPCs demonstrated little to no autofluorescence in the PE-Cy7 channel (B). Most MPCs from two representative normal individuals were positive for aggrecan (low gate: C, D) with few cells present in the high/bright gate. However, there was an equal split between low-positive and high-positive cells in the OA MPCs tested (E, F), with almost all cells positive for aggrecan staining. When data from all normal (n = 8) and OA (n = 8) patients was quantified, ~50% of OA MPCs demonstrated this abnormal aggrecan staining, while this same staining pattern was observed in ~5% of normal MPCs (G). A positive correlation was observed between cytoplasmic aggrecan staining and both K/L and synovitis score (H). *p < 0.05.

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