Fig. 2: The inhibition of piR-823 facilitates PINK1-Parkin-mediated mitophagy.
From: piR-823 inhibits cell apoptosis via modulating mitophagy by binding to PINK1 in colorectal cancer
A The colocalization of mitochondria and lysosomes stained with MitoTracker Green (MTG) and LysoTracker Red(LTR) was estimated in HCT116 and DLD-1 cell transfected with Ant-NC or Ant-823. Scale bar: 10 μm. B Formation of mitolysosomes was also observed by transmission electron microscopy imaging after piR-823 repression. Scale bar: 1 μm. C, D Western blot analysis of mitophagy-related proteins and expression of Parkin in the cytosolic and mitochondrial fractions of HCT116 and DLD-1 cells transfeced with the Ant-823 or Ant-NC for 72 h. E Mitochondrial colocation with p62 was detected by immunofluorescence. Scale bar : 10 μm. *P < 0.05, **P < 0.01 vs control groups.
