Fig. 2: The effect of EGFR expression on patient prognosis and cell proliferation. | Cell Death & Disease

Fig. 2: The effect of EGFR expression on patient prognosis and cell proliferation.

From: Inhibitory role of proguanil on the growth of bladder cancer via enhancing EGFR degradation and inhibiting its downstream signaling pathway to induce autophagy

Fig. 2

A The expression levels of EGFR in different types of bladder cancer and adjacent tissues were detected by WB. B The TCGA database was used to analyze the correlation between EGFR expression and poor prognosis in bladder cancer patients. C, D Cells were plated in 6-well plates (3.0 × 105 /well) and lysed to collect protein or RNA when density reached 80–90%, the expression of EGFR and mRNA in 4 BC cells were analyzed by WB and RT-PCR. E Cells were plated in 96-well plates (6.0 × 103 /well), The proliferation of T24 and J82 cells was detected at specified times by MTT assay. EGFR stable knockdown (F) and overexpressed (G) cell lines were constructed by lentiviral vector plasmid as described in “Materials and methods”, then cells were plated in 6-well plates (3.0 × 105 /well) and lysed to collect protein when density reached 80–90%. The expression of EGFR was detected by WB. H, I Cells were plated in 96-well plates (6.0 × 103/well), the proliferation of cell lines were detected at specified times by MTT assay. Data are representative of three independent experiments. Error bars represent means ± SD from triplicate experiments. Vehicle control means the concentration of DMSO lower than 0.3% (*P < 0.05, **P < 0.01, ***P < 0.001).

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