Fig. 3: CircTICRR knockdown promotes apoptosis via inducing autophagy in cervical cancer cells. | Cell Death & Disease

Fig. 3: CircTICRR knockdown promotes apoptosis via inducing autophagy in cervical cancer cells.

From: Oncogenic circTICRR suppresses autophagy via binding to HuR protein and stabilizing GLUD1 mRNA in cervical cancer

Fig. 3: CircTICRR knockdown promotes apoptosis via inducing autophagy in cervical cancer cells.The alternative text for this image may have been generated using AI.

A Apoptosis in SiHa and CaSki cells was analyzed by Flow Cytometry. Cells were treated with autophagy inhibitor (3-MA) or normal medium for 1 h, then combined treated with siRNAs for additional 72 h. Cell morphology (B) and autophagy-related proteins (C) were monitored by TEM and western blot, respectively. D SiHa and CaSki cells transfected with siRNAs or negative control for 72 h were treated with the autophagy inhibitor CQ or Baf-A1 for another 6 h. Then, the autophagy-related protein LC3BI/II was monitored by western blotting. E Left panel: Schematic diagram illustrating the mechanism of mRFP-GFP-LC3B. Right panel: SiHa and CaSki cells transfected with mRFP-GFP-LC3B for 24 h were transfected with si-NC, si-circTICRR#1 and #2, respectively, for another 48 h. Images were captured by confocal microscopy. Scale bar, 30 μm. Data are representative of at least three independent experiments and presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.

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