Fig. 3: Knocking-down endogenous Nkx2.8 inhibits the in vitro and vivo chemosensitivity of UC cells. | Cell Death & Disease

Fig. 3: Knocking-down endogenous Nkx2.8 inhibits the in vitro and vivo chemosensitivity of UC cells.

From: Nkx2.8 promotes chemosensitivity in bladder urothelial carcinoma via transcriptional repression of MDR1

Fig. 3: Knocking-down endogenous Nkx2.8 inhibits the in vitro and vivo chemosensitivity of UC cells.

A Western blot analysis of Nkx2.8 in Nkx2.8-silenced UC cells. B Immunofluorescence of Doxorubicin and Pirarubicin density in 5637 with scramble or Nkx2.8 knocking-down. Nuclear DNA was stained with DAPI. Scale bar, 20 μm. C CCK8 analysis of scramble or Nkx2.8 knocking-down 5637 after treated by different dose of Doxorubicin (left) or Pirarubicin (right) (mean ± SD). p values are indicated (two-tailed t test, n = 3). D Colony formation assays of scramble or Nkx2.8 knocking-down 5637 after treated by Doxorubicin or Pirarubicin (left panel) and quantification (right panel) of colonies (mean ± SD). ***p < 0.0001 (twotailed t test, n = 3). E The apoptosis rate of scramble or Nkx2.8 knocking-down 5637 after treated by Doxorubicin or Pirarubicin (mean ± SD). ***p < 0.0001 (two-tailed t test, n = 3). F Western blot analysis of apoptotic markers in scramble or Nkx2.8knocking-down 5637 after treated by Doxorubicin or Pirarubicin. G Representative images of macroscopic orthophoric bladder urothelial cancer generated from scramble cells or Nkx2.8 knocking-down 5637 before or after treated with Doxorubicin (Dox) or Pirarubicin (Pir) (left panel). Quantification of luminescence in orthophoric bladder urothelial cancer (right panel) mean ± SD. ***p < 0.0001(two-tailed t test, n = 4). H Representative images of H&E staining. Scale bar, 500 μm. I TUNEL assay of apoptosis in tumor tissues. Scale bar, 20 μm.

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