Fig. 2: Activation of cAMP compromises self-renewal and represses proliferation in GSCs. | Cell Death & Disease

Fig. 2: Activation of cAMP compromises self-renewal and represses proliferation in GSCs.

From: Disruption of β-catenin-mediated negative feedback reinforces cAMP-induced neuronal differentiation in glioma stem cells

Fig. 2

A Phase-contrast images of neurospheres formed by GSC1 and GSC11 cells after treatment with cAMP activators for 72 h. B Limiting dilution assays of GSC1 and GSC11 cells treated with dbcAMP and forskolin. C Western blot of CD133 and SOX2 protein in GSCs exposed to dbcAMP and forskolin (left) and quantification of protein bands (right). D GSEA analysis using the mRNA sequencing data. E Flow cytometry and EdU incorporation DNA histograms of GSCs before and after the treatment with dbcAMP and forskolin. (scale bar, 100 μm; data are shown as the mean ± SD. N.S., not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001).

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