Fig. 4: PTX3 has indirect effects on chondrocyte metabolism through regulation of macrophage polarization. | Cell Death & Disease

Fig. 4: PTX3 has indirect effects on chondrocyte metabolism through regulation of macrophage polarization.

From: Pentraxin 3 regulated by miR-224-5p modulates macrophage reprogramming and exacerbates osteoarthritis associated synovitis by targeting CD32

Fig. 4

A, B Safranin O staining and grade of mouse tibial plateaus cartilage explants treated with IL-1β or rmPTX3, n = 3 per group; Scale bar: 50 µm. C Relative mRNA expression levels of MMP13, COL2, and SOX9 in IL-1β- or rmPTX3-treated primary murine chondrocytes, n = 6 per group. D, E Safranin O staining and grade of mouse tibial plateaus cartilage explants co-cultured with supernatants of RAW264.7 (RAW264.7 was treated with IL-1β and rmPTX3), n = 3 per group; Scale bar: 50 µm. F Relative mRNA expression levels of MMP13, COL2 and SOX9 in primary murine chondrocytes co-cultured with supernatants of RAW264.7 (RAW264.7 was treated with IL-1β or rmPTX3), n = 6 per group. *P < 0.05, **P < 0.01, ***P < 0.001, ns not significant. Data are shown as means ± SD. Statistical analyses were conducted by Kruskal–Wallis test followed by Dunn’s multiple comparisons test (B and E) or one-way analysis of variance followed by Dunnett’s multiple comparison test (C and F).

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