Fig. 3: Low expression of GBA1 promotes the epithelial-mesenchymal transition (EMT) via activation of the Wnt/β-catenin signalling pathway. | Cell Death & Disease

Fig. 3: Low expression of GBA1 promotes the epithelial-mesenchymal transition (EMT) via activation of the Wnt/β-catenin signalling pathway.

From: GBA1-dependent membrane glucosylceramide reprogramming promotes liver cancer metastasis via activation of the Wnt/β-catenin signalling pathway

Fig. 3

A, D The expression of EMT markers in MHCC-97H cells with stable GBA1 overexpression (A) or HepG2 cells with stable GBA1 downregulation (D) as detected by Western blotting. B, E The expression of phospho-LRP6 and the Wnt target genes c-myc and/or cyclin D1, which are related to the Wnt signalling pathway in MHCC-97H cells with stable GBA1 overexpression (B) or HepG2 cells with stable GBA1 downregulation (E) was detected by Western blotting. C, F Western blot analysis was performed to determine the level of non-phospho-β-catenin in the cytoplasm and nucleus of MHCC-97H cells with stable GBA1 overexpression (C) or HepG2 cells with stable GBA1 downregulation (F). G Western blotting was performed to analyse the effect of GBA1 overexpression on the Wnt3a-activating EMT. H Western blotting was performed to analyse the effect of GBA1 overexpression on the Wnt3a-activated Wnt signalling pathway.

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