Fig. 2: STAT6 deficiency in lung epithelium aggravates CS-induced ferroptosis and lung injury.

Wild type (WT) and lung epithelium-specific STAT6 deficiency mice (STAT6^cKO) mice were intratracheally instilled with CS and sacrificed 7 days later. A Body weight (BW) and B the ratio of lung weight to BW were determined. C Representative H&E-stained lung sections from WT and STAT6^cKO mice and lung injury score. (Black and blue arrows indicated inflammatory nodules and inflammatory cells infiltration respectively). D IHC staining of 8-oxo-dG and PTGS-2 of lung sections from indicated group were performed and quantified. Representative images from each group were shown. The protein expression (E) and mRNA F levels of STAT6, PTGS-2 and SLC7A11 in lung tissue were determined by western blot analysis and qRT-PCR assay. The relative GSH G, MDA (H) and Iron I content were measured by corresponding kit. J IHC staining of 4-HNE in lung sections from indicated group were performed and quantified. Representative images from each group were shown. The data were presented as means ± SD (n = 6, *p < 0.05, Ctrl vs. CS; ^#p < 0.05, WT vs. STAT6^cKO).