Fig. 2: Elevated DcR2 exacerbates renal fibrosis in STZ-induced DN. | Cell Death & Disease

Fig. 2: Elevated DcR2 exacerbates renal fibrosis in STZ-induced DN.

From: Decoy receptor 2 mediates the apoptosis-resistant phenotype of senescent renal tubular cells and accelerates renal fibrosis in diabetic nephropathy

Fig. 2

A Experimental design. Red arrows indicate the injection of STZ (mg/kg), while green arrows indicate the transfer of pLenti-C-mGFP DcR2 overexpression or pGFP-C-shLenti DcR2-siRNA plasmids. B, C qRT-PCR results for α-SMA and collagen I mRNA levels in each group (n = 6). D, E Masson trichrome staining showing renal fibrosis (blue) and the percentage of fibrosis in different groups after quantitative analysis. Scale bar, 40 μm. F Total collagen in each group (n = 6). G Immunohistochemical staining for DcR2, α-SMA, and collagen IV. Scale bar, 40 μm. H–J. The percentage of positive RTECs was quantified (n = 6–7). Data are expressed as the mean ± SD for each group. *P < 0.05 versus control; #P < 0.05 versus STZ.

Back to article page