Fig. 2: BLM-induced lung fibrosis is suppressed by preventive TP53TG1.
From: Systematic analyses identify the anti-fibrotic role of lncRNA TP53TG1 in IPF
a Diagram of the animal experimental mode. Mice received intratracheal injection of AAV5-TP53TG1 14 days before BLM induction, and lung tissues were harvested 21 days later. b TP53TG1 was significantly overexpressed in lung of AAV5-TP53TG1 infected mice confirmed by agarose gel electrophoresis; n = 6. c The relative expression of fibrosis related genes induced by BLM was suppressed by prior injection of AAV5-TP53TG1. d The effect of TP53TG1 on the protein levels of Fn1 and Collagen I in vivo was assessed by Western blots. e Hydroxyproline content was significantly reduced in the lungs of AAV5-TP53TG1 treated mice. f H&E and Masson staining indicated that TP53TG1 inhibited BLM-induced lung morphological changes and increased fibrotic area; n = 3. g Immunohistochemical experiments detected lighter staining for Collagen I and α-SMA in the AAV5-TP53TG1-treated BLM-mice; n = 3. *P < 0.05; **P < 0.01. BLM, bleomycin; AAV-TP53TG1, adeno-associated virus 5 containing TP53TG1; FC fold change.
