Fig. 2: STX18 knockdown sensitizes radioresistant A549 and radiosensitive H460 cell lines to irradiation. | Cell Death & Disease

Fig. 2: STX18 knockdown sensitizes radioresistant A549 and radiosensitive H460 cell lines to irradiation.

From: Syntaxin 18 regulates the DNA damage response and epithelial-to-mesenchymal transition to promote radiation resistance of lung cancer

Fig. 2: STX18 knockdown sensitizes radioresistant A549 and radiosensitive H460 cell lines to irradiation.

a A549 and H460 cells were transduced with a shRNA targeting STX18 and its expression was quantified by RT-qPCR. n = 3–4. The insert shows respective immunoblotting analyses of STX18 expression. Here, quantification was achieved after normalization to the loading control (Actin) and the shScr sample. n = 3. b Proliferation of unirradiated cells was measured by proliferation/cell viability assay. n = 3. c Quantification of sub-G1 fraction by flow cytometry after irradiation. Cells were irradiated and cell cycle distribution was quantified by PI staining after 72 h. n = 3–5. d Colony formation ability was assessed after irradiation. Cells were irradiated and colonies were counted after 10 days. n = 3.

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