Fig. 1: COL18A1-AS1 was downregulated and could be a biomarker for ccRCC. | Cell Death & Disease

Fig. 1: COL18A1-AS1 was downregulated and could be a biomarker for ccRCC.

From: Restoring the epigenetically silenced lncRNA COL18A1-AS1 represses ccRCC progression by lipid browning via miR-1286/KLF12 axis

Fig. 1

A The expression level of COL18A1-AS1 in 72 normal renal tissues and 522 ccRCC tissues from TCGA database. B The expression level of COL18A1-AS1 in 71 pairs of matched ccRCC and normal tissues from TCGA database. C, D The Kaplan–Meier curves of COL18A1-AS1 in ccRCC for overall survival and disease-free survival. E–I The expression of COL18A1-AS1 was related with various clinicopathological factors. J The receiver operating characteristic (ROC) curves of COL18A1-AS1 (AUC = 0.9190; p < 0.0001). K The expression level of COL18A1-AS1 in RCC cell lines (ACHN, A498, CAKI-1, OS-RC-2, and 786-O) and normal cell line (HK-2). L The expression level of COL18A1-AS1 in 20 ccRCC tissues and adjacent normal tissues. M Localization of COL18A1-AS1 was assessed by PCR in CAKI-1 and 786-O cells. U6 and GAPDH were used as positive controls for nuclear RNA and cytoplasmic RNA, respectively. N The distribution of COL18A1-AS1 was analyzed by FISH in CAKI-1 and 786-O cells. 18 S and U6 showed cytoplasm and nucleus, respectively. Scale bars, 10 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate mean ± SD. All the experiments were performed in triplicate.

Back to article page