Fig. 3: DNA methylation caused the low expression of COL18A1-AS1 in ccRCC. | Cell Death & Disease

Fig. 3: DNA methylation caused the low expression of COL18A1-AS1 in ccRCC.

From: Restoring the epigenetically silenced lncRNA COL18A1-AS1 represses ccRCC progression by lipid browning via miR-1286/KLF12 axis

Fig. 3

A Prediction analysis of CpG islands in the sequence range of 3400 bp upstream from the transcriptional start site in the COL18A1-AS1 promoter region. B BSP results of COL18A1-AS1 methylation status in adjacent tissues and ccRCC tissues. C Heat map of methylation percentage of COL18A1-AS1 and histogram of quantitative results. D The primers sequence of MSP and representative MSP results of COL18A1-AS1 methylation status in adjacent tissues and ccRCC tissues. E The expression levels of COL18A1-AS1 in RCC cell lines after 5-AZA treatment. F The expression levels of COL18A1-AS1 in RCC cell lines after CRISPR/dCas9-mediated editing system specific demethylation. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate mean ± SD. All the experiments were performed in triplicate.

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