Fig. 1: OTUB1 augments hypoxia signaling. | Cell Death & Disease

Fig. 1: OTUB1 augments hypoxia signaling.

From: OTUB1 augments hypoxia signaling via its non-canonical ubiquitination inhibition of HIF-1α during hypoxia adaptation

Fig. 1: OTUB1 augments hypoxia signaling.The alternative text for this image may have been generated using AI.

A–D qPCR analysis of PGK1 (A), LDHA (B), VEGF (C), and BNIP3 (D) mRNA in H1299 cells transfected with or without Flag-OTUB1 and cultured under normoxia (21% O2) or hypoxia (1% O2) for 24 h. Flag empty vector was used as a control. E–H qPCR analysis of PGK1 (E), LDHA (F), PDK1 (G), and BNIP3 (H) mRNA in H1299 cells transfected with or without Flag-OTUB1 and treated with or without CoCl2 (200 μM) for 8 h. Flag empty vector was used as a control. I–L qPCR analysis of PGK1 (I), LDHA (J), PDK1 (K), and BNIP3 (L) mRNA in H1299 cells transfected with or without Flag-OTUB1 and treated with DFX (150 μM) or DMSO as a control for 8 h. Flag empty vector was used as a control. Two-way ANOVA analysis; Data show mean ± SD; Tukey’s multiple comparisons test; ns, not significant, *Adjusted p < 0.05, **Adjusted p < 0.01, ***Adjusted p < 0.001, ****Adjusted p < 0.0001; Data from 3 independent experiments.

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