Fig. 5: LKB1/AMPK/ULK1 signaling pathway is involved in Pep A-induced autophagy flux. | Cell Death & Disease

Fig. 5: LKB1/AMPK/ULK1 signaling pathway is involved in Pep A-induced autophagy flux.

From: Itch and autophagy-mediated NF-κB activation contributes to inhibition of cathepsin D-induced sensitizing effect on anticancer drugs

Fig. 5

A, B Examination of protein expression in Caki/Cat D WT and KO cells or Cont and Cat D siRNA-transfected cancer cells. C Caki cells were pretreated with the indicated concentrations of Everolimus, and 2 μM Pep A was then added for 24 h. D Caki cells were transiently transfected with Cont siRNA or AMPK siRNA, and they were treated with 2 μM Pep A for 24 h. E Caki cells were pretreated with the indicated concentrations of Compound C, and 2 μM Pep A was then added for 24 h. F Cancer cells (Caki, HT29, and DU145) cells were transiently transfected with Cont siRNA or AMPK siRNA, and they were then treated with 2 μM Pep A for 24 h. G Caki cells were transiently transfected with Cont siRNA, LKB1 siRNA, TAK1 siRNA, or CaMKKβ siRNA, and they were treated with 2 μM Pep A for 24 h. H A549 and Caki cells were treated with the indicated concentrations of Pep A for 24 h. I Caki cells were pretreated with the indicated concentrations of SP600125 for 30 min, and they were then treated with 2 μM Pep A for 24 h. Protein expression was measured via western blotting (A–I).

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