Fig. 7: Ets-1 regulates the antioxidant pathway in sorafenib resistance. | Cell Death & Disease

Fig. 7: Ets-1 regulates the antioxidant pathway in sorafenib resistance.

From: Ets1 mediates sorafenib resistance by regulating mitochondrial ROS pathway in hepatocellular carcinoma

Fig. 7

A Hep3B-soraR cells transiently transfected with control-siRNA or Ets-1-siRNA were treated with DMSO or sorafenib (6 µM) for 24 h and analyzed by flow cytometry to detect mROS, as described in Fig. 2E. The bar graphs on the right represent the degree of mROS induction under each condition. The data represent the mean ± S.D. of two independent experiments. B Hep3B-soraR cells transfected as in A were treated with DMSO or sorafenib for 36 h and subjected to mitochondrial and cytoplasmic fractionation and analyzed by western blots. Cyto C cytochrome C. C–I Hep3B-soraR cells transfected as in A were treated with DMSO or sorafenib for 24 h followed by RNA extraction and qPCR analysis of the indicated genes. The data represent the mean ± S.D. of three independent PCR reactions. In all bar graphs, lanes 1 and 2 were transfected with control-siRNA, and lanes 3 and 4 were transfected with Ets-1-siRNA and treated with DMSO (lanes 1, 3) or sorafenib (lanes 2, 4). Significant differences were determined by t test and indicated as: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.

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