Fig. 5: NONO affects the sensitivity of AML cells to Ara-C.
From: Stabilization of SAMHD1 by NONO is crucial for Ara-C resistance in AML
A IC50 values of Ara-C in AML cell lines. AML cells (1 × 105 cells/well) plated in 24-well plates were incubated for 24 h and were then treated with a series of concentrations of Ara-C for 48 h. Cell viability was evaluated by a CCK-8 assay (n = 3, mean ± SD). B, C Silencing NONO increased the sensitivity of AML cells to Ara-C. THP-1 and MV4-11 cells with stable knockdown of NONO were treated with different concentrations of Ara-C for 48 h, and cell viability was evaluated by a CCK-8 assay (n = 3, mean ± SD). D, E Overexpression of NONO decreased the sensitivity of AML cells to Ara-C. HL60 and HEL cell lines with stable overexpression of NONO were generated and treated with different concentrations of Ara-C for 48 h. Cell viability was evaluated by a CCK-8 assay (n = 3, mean ± SD). B–E, two-way ANOVA was used to compare the behavior of NONO-silenced or overexpressed and control cells. For respective immunoblots, the protein levels were quantified by ImageJ software.
