Fig. 4: RNA-seq analysis following DHODH inhibition in CML.
From: Dihydroorotate dehydrogenase inhibition reveals metabolic vulnerability in chronic myeloid leukemia
A The volcano plot demonstrates the number of up- and down-regulated genes in CML CD34+ (n:5) patients’ cells after treatment with 100 nM Meds433 for 3 days. B The top 100 differentially expressed genes based on adjusted p value are depicted in the heatmap, and C shows enriched and depleted hallmark gene sets and three enrichment plots related to apoptosis, P53 pathway, and MYC targets. The number in the plots demonstrates a normalized enrichment score (NES). D demonstrates Western blot analysis of JURL-MK1 cell line treated with 100 nM Meds433 for 3 days (n:3). E shows CFU assay after the treatment of CML CD34+ (n:5) patients’ cells with Meds433. For this figure, the total number of colonies (CFU-GM + CFU-E + BFU-E) and the number of each subset are displayed. *p < 0.05, **p < 0.01, ***p < 0.001.
