Fig. 3: caPAK4 reduces α-synuclein aggregates in the A53T PD model. | Cell Death & Disease

Fig. 3: caPAK4 reduces α-synuclein aggregates in the A53T PD model.

From: p21-activated kinase 4 controls the aggregation of α-synuclein by reducing the monomeric and aggregated forms of α-synuclein: involvement of the E3 ubiquitin ligase NEDD4-1

Fig. 3: caPAK4 reduces α-synuclein aggregates in the A53T PD model.The alternative text for this image may have been generated using AI.

A Double immunostaining for pPAK4 (red) and TH (green). Scale bar, 100 µm. B Quantification of pPAK4 staining intensity in TH-positive dopamine neurons (a.u., arbitrary units) (n = 5 for each group). C Experimental scheme. D Expression of caPAK4 (GFP; green) in TH-positive dopamine neurons of A53T-Tg mice. E–H Representative images of α-synuclein immunostaining in the SN of A53T-Tg mice injected with lenti-Ctrl or lenti-caPAK4. Scale bar, 100 µm. I Quantification of the intensity of total α-synuclein (E, F) in SN (n = 5 for each group). J Quantification of the number of proteinase K-resistant α-synuclein inclusions (G, H) in SN (n = 5 for each group). K, L Representative images of pα-synS129 immunostaining in the SN of A53T-Tg mice injected with lenti-Ctrl or lenti-caPAK4. Scale bar, 100 µm. M Quantification of the number of pα-synS129-positive cells in SN. Arrowheads and arrows indicate granular deposits and neurites, respectively. The data are presented as the mean ± SEM. *P < 0.01, **P < 0.01. Unpaired Student’s t test.

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