Fig. 4: caPAK4 reduces α-synuclein aggregates in the AAV-hα-syn-induced PD model. | Cell Death & Disease

Fig. 4: caPAK4 reduces α-synuclein aggregates in the AAV-hα-syn-induced PD model.

From: p21-activated kinase 4 controls the aggregation of α-synuclein by reducing the monomeric and aggregated forms of α-synuclein: involvement of the E3 ubiquitin ligase NEDD4-1

Fig. 4: caPAK4 reduces α-synuclein aggregates in the AAV-hα-syn-induced PD model.The alternative text for this image may have been generated using AI.

A Experimental scheme. B–E Representative images of α-synuclein immunostaining in the SN of lenti-Ctrl- or lenti-caPAK4-injected rats post AAV-hα-syn. Arrowheads, α-synuclein aggregates; arrow, Lewy-like neurites. Scale bar, 100 µm. F Quantification of the intensity of total α-synuclein (B, C) in SN (n = 5 mice per group). G Quantification of the number of proteinase K-resistant α-synuclein inclusions (D, E) in the SN (n = 5 mice per group). H Representative immunoblots for α-synuclein in Triton X-100-insoluble and soluble fractions of the SN from lenti-Ctrl- or lenti-caPAK4-injected rats post AAV-hα-syn. I Quantification of the density of α-synuclein in Triton X-100-insoluble and soluble fractions normalized to β-actin (n = 5 for each group). J, K Representative images of pα-synS129 immunostaining in the SN of lenti-Ctrl- or lenti-caPAK4-injected rats post AAV-hα-syn. Scale bar, 100 µm. L Quantification of the number of pα-synS129-positive cells in the SN. Arrowheads, granular deposits; arrows, neurites. The data are presented as the mean ± SEM. *P < 0.01. Unpaired Student’s t test.

Back to article page