Fig. 7: PAK4 enhances the association of NEDD4-1 with α-synuclein through phosphorylation of NEDD4-1. | Cell Death & Disease

Fig. 7: PAK4 enhances the association of NEDD4-1 with α-synuclein through phosphorylation of NEDD4-1.

From: p21-activated kinase 4 controls the aggregation of α-synuclein by reducing the monomeric and aggregated forms of α-synuclein: involvement of the E3 ubiquitin ligase NEDD4-1

Fig. 7: PAK4 enhances the association of NEDD4-1 with α-synuclein through phosphorylation of NEDD4-1.The alternative text for this image may have been generated using AI.

A In vitro kinase assay for PAK4-mediated phosphorylation of NEDD4-1 with vehicle (control) or PF3758309 (10 μM). C-PAK4, C-terminal kinase domain of PAK4. B Immunoblotting for NEDD4-1 with anti-phospho-serine/threonine (α-pS/T) and anti-phospho-tyrosine (α-pY) antibodies in vector- or myc-caPAK4–transfected cells. C Immunoblotting for NEDD4-1 in vector- or myc-caPAK4–transfected cells over 24 h following treatment with cycloheximide (CHX; 20 μg/ml). D In vitro ubiquitination assay to assess the catalytic activity of NEDD4-1 with vehicle (control) or PF3758309 (10 μM). E Immunoblotting after immunoprecipitation with anti-HA (NEDD4-1) with vehicle (control) or PF3758309 (10 μM). Each blot is representative of at least two experiments.

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