Fig. 5: sGRP78 promotes autophagosome formation in myeloid cells.
From: sGRP78 enhances selective autophagy of monomeric TLR4 to regulate myeloid cell death
A–D 12 h after sGRP78 treatment, BMDCs (A, C) and BMDMs (B, D) were immunolabeled with LC3 (A, B) and p62 (C, D) antibody. BMDCs (E–H) or FITC-LPS-treated BMDCs (I) were incubated with sGRP78 for 1 h, followed by detection of intracellular LC3, p62, LPS, TLR4, and LAMP1. The colocalization between two markers was described by 2D intensity histogram and Pearson's R value. All images are representative of at least three independent experiments in which >100 cells were examined, and >95% of cells showed similar staining. Scale bar = 10 μm (A–D) or 2 μm (E–H). **P < 0.01, ****P < 0.0001.
