Fig. 3: The inhibition of metastasis by CYB5A is related to its ability to increase autophagy. | Cell Death & Disease

Fig. 3: The inhibition of metastasis by CYB5A is related to its ability to increase autophagy.

From: Cytochrome B5 type A alleviates HCC metastasis via regulating STOML2 related autophagy and promoting sensitivity to ruxolitinib

Fig. 3: The inhibition of metastasis by CYB5A is related to its ability to increase autophagy.

A The different expression genes under normoxia between HCCLM3-control and HCCLM3-CYB5A cell lines. The significant thresholds were |fold change | > 2 and p < 0.05. Significant different expression genes were filtered and enriched in GO TRRUST analysis and KEGG signaling pathway. B, C After the cells were fixed by paraformaldehyde, LC3 expression was detected by immunofluorescence (IF) and immunocytochemistry (ICC). D Cell lines treated with or without 100 nM bafilomycin A1 (Baf A1) for 4 h. Cell lysates were analyzed by western blotting. E Images represented the typical transmission electron microscopy of indicated cell lines in the top panel and statistical analysis of autophagolysosome (ASS) numbers are in the bottom panel. The red arrows indicate ASS. F Images represented the typically indicated cell lines that stably expressed mRFP-EGFP-LC3 fusion protein which was shown by confocal microscopic analysis in the top panel and statistical analysis of autophagosome and ASS numbers in the bottom panel. Scale bar: 20 μm.

Back to article page